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  Indian J Med Microbiol
 

Figure 6: O. Indicum in combination with gemcitabine, anticancer drug, effects on cytotoxicity, apoptosis, and ROS formation in CCA cells. (a) KKU-100 cells were exposed to O. Indicum (0-250 μM), gemcitabine (0-25 mM), and in combination of O. Indicum (50 μg/mL), gemcitabine (0-25 mM), for 24 h and proliferation was investigated using a SRB assay. (b) KKU-100 cells were incubated with O. Indicum (50 μg/mL) in combination with gemcitabine (10 mM), stained with Annexin-V FITC/PI solution, apoptosis was measured by flow cytometry. (c) KKU-100 cells were treated, stained with DCF-DA solution, ROS formation was measured by flow cytometry. Mean ± SE (n = 3). *P < 0.05

Figure 6: <i>O. Indicum</i> in combination with gemcitabine, anticancer drug, effects on cytotoxicity, apoptosis, and ROS formation in CCA cells. (a) KKU-100 cells were exposed to <i>O. Indicum</i> (0-250 μM), gemcitabine (0-25 mM), and in combination of <i>O. Indicum</i> (50 μg/mL), gemcitabine (0-25 mM), for 24 h and proliferation was investigated using a SRB assay. (b) KKU-100 cells were incubated with <i>O. Indicum</i> (50 μg/mL) in combination with gemcitabine (10 mM), stained with Annexin-V FITC/PI solution, apoptosis was measured by flow cytometry. (c) KKU-100 cells were treated, stained with DCF-DA solution, ROS formation was measured by flow cytometry. Mean ± SE (<i>n = 3</i>). <i>*P</i> < 0.05