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  Indian J Med Microbiol
 

Figure 4: Effects of tanshinone IIA on GSK-3β phosphorylation of SH-SY5Y cells injured by Aβ25–35. After treating with sodium 4-phenylbutyrate or indicated concentrations of tanshinone IIA on Aβ25–35-injured SH-SY5Y cells, (a) phosphorylation levels of GSK-3β protein were measured by immunofluorescence and (b) protein levels of phosphorylated GSK-3β proteins (Y216 and S9) and total GSK-3β proteins were measured by Western BLOT. Statistical data are presented as mean ± standard deviation n = 3, **P < 0.01, versus control group. #P < 0.05, ##P < 0.01, versus Aβ group. The experiments were independently repeated three times

Figure 4: Effects of tanshinone IIA on GSK-3β phosphorylation of SH-SY5Y cells injured by Aβ<sub>25–35</sub>. After treating with sodium 4-phenylbutyrate or indicated concentrations of tanshinone IIA on Aβ<sub>25–35</sub>-injured SH-SY5Y cells, (a) phosphorylation levels of GSK-3β protein were measured by immunofluorescence and (b) protein levels of phosphorylated GSK-3β proteins (Y216 and S9) and total GSK-3β proteins were measured by Western BLOT. Statistical data are presented as mean ± standard deviation <i>n</i> = 3, **<i>P</i> < 0.01, versus control group. <sup>#</sup><i>P</i> < 0.05, <sup>##</sup><i>P</i> < 0.01, versus Aβ group. The experiments were independently repeated three times