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  Indian J Med Microbiol
 

Figure 5: HO-1 mediates the suppressive effects of CBTM-E375 on LPS-stimulated pro-inflammatory cytokines production. HUVEC cells were pretreated for 3 h with CBTM-E375 (400 μg/mL) in the presence or absence of small interfering RNA HO-1(40 μM) and stimulated for 24 h with LPS (1 μg/mL), the HO-1 expression was measured (a) and the levels of IL-6 (b), IL-8 (c) and TNF-α (d) were detected by ELISA. Data represent mean values of 3 experiments ± standard deviation. #P < 0.05 versus control group; ##P < 0.01 versus control group; δP < 0.05 versus control group; δδP < 0.01 versus CBTM-E375 group *P < 0.05 versus LPS group; P < 0.05 versus LPS + CBTM-E375 group was considered to be statistically significant. HUVECs: Human umbilical vein endothelial cells; HO-1: Heme Oxygenase-1; LPS: Lipopolysaccharide

Figure 5: HO-1 mediates the suppressive effects of CBTM-E375 on LPS-stimulated pro-inflammatory cytokines production. HUVEC cells were pretreated for 3 h with CBTM-E375 (400 μg/mL) in the presence or absence of small interfering RNA HO-1(40 μM) and stimulated for 24 h with LPS (1 μg/mL), the HO-1 expression was measured (a) and the levels of IL-6 (b), IL-8 (c) and TNF-α (d) were detected by ELISA. Data represent mean values of 3 experiments ± standard deviation. <sup>#</sup><i>P</i> < 0.05 versus control group; <sup>##</sup><i>P</i> < 0.01 versus control group; <sup>δ</sup><i>P</i> < 0.05 versus control group; <sup>δδ</sup><i>P</i> < 0.01 versus CBTM-E375 group *<i>P</i> < 0.05 versus LPS group; <sup>†</sup><i>P</i> < 0.05 versus LPS + CBTM-E375 group was considered to be statistically significant. HUVECs: Human umbilical vein endothelial cells; HO-1: Heme Oxygenase-1; LPS: Lipopolysaccharide