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  Indian J Med Microbiol
 

Figure 5: Photomicrograph of thoracic aortas represent immunohistochemical staining with RAM-11 antibody from CN, HCD, ZI, ZII, ZIII, SG and ZSG groups. CN shows no IPC. HCD displays massive and diffuse IPCs within thick rising plaque (p) indicated by deep brownish-stained macrophages (black and yellow arrows). Aortas in ZPI, ZPII, and ZPIII groups demonstrate a significant reduction in plaque size (p) and the intensity of brownish color RAM-11 immunopositive foam cells compare to the thick plaque in HCD (black arrows). There is no reaction in the muscularis layer (m). However, ZSG group compared to SG group had shown a significant reduction in macrophage expression within the intimal plaque. Bar graph represents the quantification assay of IPC and INC. *indicates statistical significance compared to HCD at P < 0.05. L: Lumen; M: Media; A: Adventitia. Scale bars: 200 μm. CN: Control group; RAM-11: Regulation of ACE2 and morphogenesis 11; ZP: ZER-preventive; ZER: Zerumbone; IPC: Immunoreactive-positive cells; INC: Immunoreactive-negative cells

Figure 5: Photomicrograph of thoracic aortas represent immunohistochemical staining with RAM-11 antibody from CN, HCD, ZI, ZII, ZIII, SG and ZSG groups. CN shows no IPC. HCD displays massive and diffuse IPCs within thick rising plaque (p) indicated by deep brownish-stained macrophages (black and yellow arrows). Aortas in ZPI, ZPII, and ZPIII groups demonstrate a significant reduction in plaque size (p) and the intensity of brownish color RAM-11 immunopositive foam cells compare to the thick plaque in HCD (black arrows). There is no reaction in the muscularis layer (m). However, ZSG group compared to SG group had shown a significant reduction in macrophage expression within the intimal plaque. Bar graph represents the quantification assay of IPC and INC. *indicates statistical significance compared to HCD at<i> P </i>< 0.05. L: Lumen; M: Media; A: Adventitia. Scale bars: 200 μm. CN: Control group; RAM-11: Regulation of ACE2 and morphogenesis 11; ZP: ZER-preventive; ZER: Zerumbone; IPC: Immunoreactive-positive cells; INC: Immunoreactive-negative cells