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  Indian J Med Microbiol
 

Figure 5: Activation of caspases and degradation of PARP [poly (ADP-ribose) polymerase] protein in AGS cells following CF treatment. (a) Changes in caspase-3 and-9 and PARP cleavage activity were investigated by western blotting. (b) AGS cells were lysed and these lysates were assayed for caspase-3 and -9 activities. (c) Pretreatment with z-VAD-fmk significantly suppressed CF-induced apoptosis of AGS cells. Results are presented as the means ± standard error means. *P < 0.05, **P < 0.01 versus untreated controls. GAPDH was used as the loading control. CF, Carthami flos; CTRL, control

Figure 5: Activation of caspases and degradation of PARP [poly (ADP-ribose) polymerase] protein in AGS cells following CF treatment. (a) Changes in caspase-3 and-9 and PARP cleavage activity were investigated by western blotting. (b) AGS cells were lysed and these lysates were assayed for caspase-3 and -9 activities. (c) Pretreatment with z-VAD-fmk significantly suppressed CF-induced apoptosis of AGS cells. Results are presented as the means ± standard error means. *<i>P</i> < 0.05, **<i>P</i> < 0.01 versus untreated controls. GAPDH was used as the loading control. CF, <i>Carthami flos</i>; CTRL, control