Close
  Indian J Med Microbiol
 

Figure 5: Neuroprotective effect of alkaloid neferine on inducible nitric oxide synthase, cylcooxygenase-2 and TH protein expression in the substantia nigra tissue of Parkinson's disease-induced mice. The mice were subjected to Parkinson's disease induction with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine with 1-methyl-4-phenyl-1,2, 3,6-tetrahydropyridine (30 mg/kg bw) intraperitoneally for 5 consecutive days, nefereine (20 mg/kg bw) intraperitonial injection was given from day 10 to 14 of treatment period to Parkinson's disease-induced mice and nefereine (20 mg/kg bw) intraperitonial injection alone was administered for 14 days. 24 h after the treatment period the mice were euthanized and the substantia nigra was isolated from dissected brain of control and experimental mice. The tissue was lysed with RIPA buffer, centrifuged at 1200 rpm for 15 min and the supernatant was subjected to protein estimation. 40 μg of total protein from control and experimental mice samples were subjected to electrophoresis and immublotting analysis with specific proteins inducible nitric oxide synthase, cylcooxygenase-2 and TH protein. The protein bands were visualized using enzyme chemiluminescence kit and representative image were depicted

Figure 5: Neuroprotective effect of alkaloid neferine on inducible nitric oxide synthase, cylcooxygenase-2 and TH protein expression in the substantia nigra tissue of Parkinson's disease-induced mice. The mice were subjected to Parkinson's disease induction with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine with 1-methyl-4-phenyl-1,2, 3,6-tetrahydropyridine (30 mg/kg bw) intraperitoneally for 5 consecutive days, nefereine (20 mg/kg bw) intraperitonial injection was given from day 10 to 14 of treatment period to Parkinson's disease-induced mice and nefereine (20 mg/kg bw) intraperitonial injection alone was administered for 14 days. 24 h after the treatment period the mice were euthanized and the substantia nigra was isolated from dissected brain of control and experimental mice. The tissue was lysed with RIPA buffer, centrifuged at 1200 rpm for 15 min and the supernatant was subjected to protein estimation. 40 μg of total protein from control and experimental mice samples were subjected to electrophoresis and immublotting analysis with specific proteins inducible nitric oxide synthase, cylcooxygenase-2 and TH protein. The protein bands were visualized using enzyme chemiluminescence kit and representative image were depicted