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  Indian J Med Microbiol
 

Figure 4: Anti-inflammatory effect of alkaloid neferine on Parkinson's disease-induced mice proinflammatory cytokine levels in the substantia nigra tissue. The mice were subjected to Parkinson's disease induction with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (30 mg/kg bw) intraperitoneally for 5 consecutive days, nefereine (20 mg/kg bw) intraperitonial injection was given from day 10 to 14 of treatment period to Parkinson's disease-induced mice and nefereine (20 mg/kg bw) intraperitonial injection alone was administered for 14 days. 24 h after the treatment period the mice were euthanized and the substantia nigra was isolated from dissected brain of control and experimental mice. The tissue was lysed with homogenizing buffer and subjected to measurement of cytokines tumor necrosis factor-α, interleukin-1β and interleukin-6 levels using commercially available ELISA kit. The data were statistically analyzed with one way analysis of variance followed by Student–Newman–Keuls post hoc test using GraphPad Prism software and expressed as mean ± standard error of the mean. P < 0.05 was considered to statistically significant

Figure 4: Anti-inflammatory effect of alkaloid neferine on Parkinson's disease-induced mice proinflammatory cytokine levels in the substantia nigra tissue. The mice were subjected to Parkinson's disease induction with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (30 mg/kg bw) intraperitoneally for 5 consecutive days, nefereine (20 mg/kg bw) intraperitonial injection was given from day 10 to 14 of treatment period to Parkinson's disease-induced mice and nefereine (20 mg/kg bw) intraperitonial injection alone was administered for 14 days. 24 h after the treatment period the mice were euthanized and the substantia nigra was isolated from dissected brain of control and experimental mice. The tissue was lysed with homogenizing buffer and subjected to measurement of cytokines tumor necrosis factor-α, interleukin-1β and interleukin-6 levels using commercially available ELISA kit. The data were statistically analyzed with one way analysis of variance followed by Student–Newman–Keuls <i>post hoc</i> test using GraphPad Prism software and expressed as mean ± standard error of the mean. <i>P</i> < 0.05 was considered to statistically significant