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  Indian J Med Microbiol
 

Figure 4: Suppressive effects of Rd on Promatrix metalloproteinase-2 at activity (a) and protein (b) levels in HaCaT keratinocytes under ultraviolet-B irradiation. HaCaT cells were pretreated with Rd (0, 5, 12, and 30 μM) for 30 min prior to the irradiation. In (a), the gelatinolytic activities of Promatrix metalloproteinase-2 in conditioned medium were expressed as % of non-treated control in the lower panel, after the band strengths were determined with densitometry. In (b), the Promatrix metalloproteinase-2 proteins were determined by the Western blot analysis using anti-metalloproteinase-2 antibody. Representatives of the three independent experiments are shown. **P < 0.01; ***P < 0.001 versus ultraviolet-B irradiation alone (at column width)

Figure 4: Suppressive effects of Rd on Promatrix metalloproteinase-2 at activity (a) and protein (b) levels in HaCaT keratinocytes under ultraviolet-B irradiation. HaCaT cells were pretreated with Rd (0, 5, 12, and 30 μM) for 30 min prior to the irradiation. In (a), the gelatinolytic activities of Promatrix metalloproteinase-2 in conditioned medium were expressed as % of non-treated control in the lower panel, after the band strengths were determined with densitometry. In (b), the Promatrix metalloproteinase-2 proteins were determined by the Western blot analysis using anti-metalloproteinase-2 antibody. Representatives of the three independent experiments are shown. **<i>P</i> < 0.01; ***<i>P</i> < 0.001 versus ultraviolet-B irradiation alone (at column width)