Close
  Indian J Med Microbiol
 

Figure 4: Hairy roots intiation in various infection time duration and its biomass accumulation (a) Optimization of different infection time duration on the transformation of explants through Agrobacterium rhizogenes ATCC 15834 (b) Optimization of media on biomass accumulation from hairy root cultures of Ocimum sanctum infected with ATCC 15834. Each treatment has five replicates with 20 explants in each set of experiment. Data represent mean percentage (±) standard deviation of these replicates. a: Superscript is each value shows the significant differences (P < 0.001), b: Superscript is each value shows the significant difference (P < 0.01), c: Superscript is each value shows the significant difference (P < 0.05). Control refers to the untransformed roots which has undergone same treatment but without infection through Agrobacterium shoot tips without supplementation of hormones in the respective media. *The data were retrieved after 30 days of culture in respective manner. Reproduction size: (at column width)

Figure 4: Hairy roots intiation in various infection time duration and its biomass accumulation (a) Optimization of different infection time duration on the transformation of explants through <i>Agrobacterium rhizogenes</i> ATCC 15834 (b) Optimization of media on biomass accumulation from hairy root cultures of <i>Ocimum sanctum</i> infected with ATCC 15834. Each treatment has five replicates with 20 explants in each set of experiment. Data represent mean percentage (±) standard deviation of these replicates. a: Superscript is each value shows the significant differences (<i>P</i> < 0.001), b: Superscript is each value shows the significant difference (<i>P</i> < 0.01), c: Superscript is each value shows the significant difference (<i>P</i> < 0.05). Control refers to the untransformed roots which has undergone same treatment but without infection through <i>Agrobacterium</i> shoot tips without supplementation of hormones in the respective media. *The data were retrieved after 30 days of culture in respective manner. Reproduction size: (at column width)