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  Indian J Med Microbiol
 

Figure 7: Anti-arthritic effect of neferine on inflammatory signaling molecules in complete Freud's adjuvant-treated arthritis-induced rats. The ankle joint tissue collected from inflamed site was lysed with RIPA buffer, centrifuged at 1200 rpm for 15 min and the supernatant was subjected to protein estimation. 40 μg of total protein from control and experimental rat samples were subjected to electrophoresis and immunoblotting analysis with specific proteins cyclooxygenase-2, inducible nitric oxide synthase, nuclear factor kappa B, phosphorylation of IκB kinase α, and IκB kinase α signaling protein. The protein bands were visualized using enzyme chemiluminescence kit and representative images were depicted. #P < 0.05 when compared with the control group and *P < 0.05 when compared with arthritis-induced group

Figure 7: Anti-arthritic effect of neferine on inflammatory signaling molecules in complete Freud's adjuvant-treated arthritis-induced rats. The ankle joint tissue collected from inflamed site was lysed with RIPA buffer, centrifuged at 1200 rpm for 15 min and the supernatant was subjected to protein estimation. 40 μg of total protein from control and experimental rat samples were subjected to electrophoresis and immunoblotting analysis with specific proteins cyclooxygenase-2, inducible nitric oxide synthase, nuclear factor kappa B, phosphorylation of IκB kinase α, and IκB kinase α signaling protein. The protein bands were visualized using enzyme chemiluminescence kit and representative images were depicted. <sup>#</sup><i>P</i> < 0.05 when compared with the control group and *<i>P</i> < 0.05 when compared with arthritis-induced group