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  Indian J Med Microbiol
 

Figure 6: Effects of MCE on LPS-induced ER stress and autophagy. (a) Western blot was used to detect IRE1α phosphorylation in the cell lysates. After determining the intensity of each band using an imaging densitometer, the relative levels of IRE1α and p-IRE1α protein were calculated based on the band intensity of β-actin protein as endogenous control. The relative phosphorylation levels of IRE1α and p-IRE1α in MCE-treated cells were calculated based on the ratio of phosphorylated and nonphosphorylated protein. Data represent the mean ± SD of duplicates.*P < 0.05 versus No treatment group,#P < 0.05 versus vehicle + LPS-treated group. (b) LC3 fluorescence was detected in MCE + LPS-treated RAW264.7 cells using the Autophagy LC3-antibody-based kit for FACS analysis. The value of fluorescence intensity is taken from the representative histograms of FACS and indicated in the right corner of each panel. *P < 0.05 versus No treatment group,#P < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of Capparis ecuadorica leaves, LPS: Lipopolysaccharide, ER: Endoplasmic reticulum, SD: Standard deviation

Figure 6: Effects of MCE on LPS-induced ER stress and autophagy. (a) Western blot was used to detect IRE1α phosphorylation in the cell lysates. After determining the intensity of each band using an imaging densitometer, the relative levels of IRE1α and p-IRE1α protein were calculated based on the band intensity of β-actin protein as endogenous control. The relative phosphorylation levels of IRE1α and p-IRE1α in MCE-treated cells were calculated based on the ratio of phosphorylated and nonphosphorylated protein. Data represent the mean ± SD of duplicates.*<i>P</i> < 0.05 versus No treatment group,<sup>#</sup><i>P</i> < 0.05 versus vehicle + LPS-treated group. (b) LC3 fluorescence was detected in MCE + LPS-treated RAW264.7 cells using the Autophagy LC3-antibody-based kit for FACS analysis. The value of fluorescence intensity is taken from the representative histograms of FACS and indicated in the right corner of each panel. *<i>P</i> < 0.05 versus No treatment group,<sup>#</sup><i>P</i> < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of <i>Capparis ecuadorica</i> leaves, LPS: Lipopolysaccharide, ER: Endoplasmic reticulum, SD: Standard deviation