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  Indian J Med Microbiol
 

Figure 5: Effect of MCE on LPS-induced MAP kinases. Western blot was used to detect ERK, JNK, and p38 phosphorylation in the cell lysates. After determining the intensity of each band using an imaging densitometer, the relative levels of ERK, p-ERK, JNK, p-JNK, p38, and p-p38 protein were calculated based on the band intensity of β-actin protein as endogenous control. The relative phosphorylation levels of ERK, JNK, and p38 in MCE-treated cells were calculated based on the ratio of phosphorylated and nonphosphorylated protein. Data represent the mean ± SD of duplicates. *P < 0.05 versus No treatment group,#P < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of Capparis ecuadorica leaves; LPS: Lipopolysaccharide; SD: Standard deviation

Figure 5: Effect of MCE on LPS-induced MAP kinases. Western blot was used to detect ERK, JNK, and p38 phosphorylation in the cell lysates. After determining the intensity of each band using an imaging densitometer, the relative levels of ERK, p-ERK, JNK, p-JNK, p38, and p-p38 protein were calculated based on the band intensity of β-actin protein as endogenous control. The relative phosphorylation levels of ERK, JNK, and p38 in MCE-treated cells were calculated based on the ratio of phosphorylated and nonphosphorylated protein. Data represent the mean ± SD of duplicates. *<i>P</i> < 0.05 versus No treatment group,<sup>#</sup><i>P</i> < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of <i>Capparis ecuadorica</i> leaves; LPS: Lipopolysaccharide; SD: Standard deviation