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  Indian J Med Microbiol
 

Figure 4: Effect of MCE on inflammatory mediators in LPS-induced inflammatory response. (a) The expression levels of COX-2 and iNOS mRNA were determined by RT-PCR analyses. After determining the intensity of each band using an imaging densitometer, the relative levels of COX-2 and iNOS mRNA were calculated based on the band intensity of β-actin mRNA as endogenous control. Data are presented as the mean ± SD of duplicates. * P < 0.05 versus control group,# P < 0.05 versus vehicle group. (b) Western blot was used to detect IκBα phosphorylation in the cell lysates. After determining the intensity of each band using an imaging densitometer, the relative levels of IκBα and p-IκBα protein were calculated based on the band intensity of β-actin protein as endogenous control. The relative phosphorylation levels of IκBα in MCE-treated cells were calculated based on the ratio of phosphorylated and nonphosphorylated IκBα protein. Data represent the mean ± SD of duplicates. *P < 0.05 versus No treatment group,#P < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of Capparis ecuadorica leaves; LPS: Lipopolysaccharide; RT-PCR: Real-time-polymerase chain reaction; COX-2: Cyclooxygenase-2; iNOS: Inducible nitric oxide synthase; SD: Standard deviation

Figure 4: Effect of MCE on inflammatory mediators in LPS-induced inflammatory response. (a) The expression levels of COX-2 and iNOS mRNA were determined by RT-PCR analyses. After determining the intensity of each band using an imaging densitometer, the relative levels of COX-2 and iNOS mRNA were calculated based on the band intensity of β-actin mRNA as endogenous control. Data are presented as the mean ± SD of duplicates. * <i>P</i> < 0.05 versus control group,<sup>#</sup> <i>P</i> < 0.05 versus vehicle group. (b) Western blot was used to detect IκBα phosphorylation in the cell lysates. After determining the intensity of each band using an imaging densitometer, the relative levels of IκBα and p-IκBα protein were calculated based on the band intensity of β-actin protein as endogenous control. The relative phosphorylation levels of IκBα in MCE-treated cells were calculated based on the ratio of phosphorylated and nonphosphorylated IκBα protein. Data represent the mean ± SD of duplicates. *<i>P</i> < 0.05 versus No treatment group,<sup>#</sup><i>P</i> < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of <i>Capparis ecuadorica</i> leaves; LPS: Lipopolysaccharide; RT-PCR: Real-time-polymerase chain reaction; COX-2: Cyclooxygenase-2; iNOS: Inducible nitric oxide synthase; SD: Standard deviation