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  Indian J Med Microbiol
 

Figure 3: Effect of MCE on oxidative stress in LPS-induced inflammatory response. (a) After DCF-DA treatment, the intensity for green fluorescence in RAW264.7 cells of subset groups was detected at 200× using a fluorescence microscope (Eclipse TX100, Nikon, Tokyo, Japan). (b) After counting total number of DCF-DA stained cells per specific area, the relative level of stained cells in MCE-treated groups was represented based on the stained cell number in No treatment group. Data represent the mean ± SD of duplicates. *P < 0.05 versus No treatment group,#P < 0.05 versus vehicle + LPS-treated group. (c) RAW 264.7 cells (5 × 105 cells/ml) were treated with the vehicle, Asp, or the indicated concentrations of MCE in the absence or presence of LPS (1 μg/ml) for 24 h. After collecting the culture supernatants, NO concentration was measured using Griess reagent. Data represent the mean ± SD of duplicates. *P < 0.05 versus No treatment group,#P < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of Capparis ecuadorica leaves; LPS: Lipopolysaccharide; DCF-DA: 2',7'-Dichlorofluorescein diacetate; NO: Nitric oxide; SD: Standard deviation; Asp: Aspirin

Figure 3: Effect of MCE on oxidative stress in LPS-induced inflammatory response. (a) After DCF-DA treatment, the intensity for green fluorescence in RAW264.7 cells of subset groups was detected at 200× using a fluorescence microscope (Eclipse TX100, Nikon, Tokyo, Japan). (b) After counting total number of DCF-DA stained cells per specific area, the relative level of stained cells in MCE-treated groups was represented based on the stained cell number in No treatment group. Data represent the mean ± SD of duplicates. *<i>P</i> < 0.05 versus No treatment group,<sup>#</sup><i>P</i> < 0.05 versus vehicle + LPS-treated group. (c) RAW 264.7 cells (5 × 10<sup>5</sup> cells/ml) were treated with the vehicle, Asp, or the indicated concentrations of MCE in the absence or presence of LPS (1 μg/ml) for 24 h. After collecting the culture supernatants, NO concentration was measured using Griess reagent. Data represent the mean ± SD of duplicates. *<i>P</i> < 0.05 versus No treatment group,<sup>#</sup><i>P</i> < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of <i>Capparis ecuadorica</i> leaves; LPS: Lipopolysaccharide; DCF-DA: 2',7'-Dichlorofluorescein diacetate; NO: Nitric oxide; SD: Standard deviation; Asp: Aspirin