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  Indian J Med Microbiol
 

Figure 2: Effects of MCE on LPS-induced cytokine production. (a) LPS-stimulated RAW264.7 cells were pretreated with vehicle, Asp, or varying concentration of MCE for 2 h, and the expression levels of TNF-α, IL-1β, and IL-6 mRNA were determined by RT-PCR. After the intensity of each band was determined using an imaging densitometer, the relative levels of three genes' mRNA were calculated based on the band intensity of β-actin as endogenous control. *P < 0.05 versus No treatment group,#P < 0.05 versus vehicle + LPS-treated group. (b) The level of IL-6 protein in culture supernatants of MCE + LPS-treated RAW264.7 cells was measured by ELISA. Data represent the mean ± SD from duplicates. * P < 0.05 versus No treatment group,# P < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of C. ecuadorica leaves; LPS: Lipopolysaccharide; TNF-α: Tumor necrosis factor-α; IL: Interleukin; RT-PCR: Real-time polymerase chain reaction; SD: Standard deviation; Asp: Aspirin; ELISA: Enzyme-linked immunosorbent assay

Figure 2: Effects of MCE on LPS-induced cytokine production. (a) LPS-stimulated RAW264.7 cells were pretreated with vehicle, Asp, or varying concentration of MCE for 2 h, and the expression levels of TNF-α, IL-1β, and IL-6 mRNA were determined by RT-PCR. After the intensity of each band was determined using an imaging densitometer, the relative levels of three genes' mRNA were calculated based on the band intensity of β-actin as endogenous control. *<i>P</i> < 0.05 versus No treatment group,<sup>#</sup><i>P</i> < 0.05 versus vehicle + LPS-treated group. (b) The level of IL-6 protein in culture supernatants of MCE + LPS-treated RAW264.7 cells was measured by ELISA. Data represent the mean ± SD from duplicates. * <i>P</i> < 0.05 versus No treatment group,<sup>#</sup> <i>P</i> < 0.05 versus vehicle + LPS-treated group. MCE: Methanolic extract of <i>C. ecuadorica</i> leaves; LPS: Lipopolysaccharide; TNF-α: Tumor necrosis factor-α; IL: Interleukin; RT-PCR: Real-time polymerase chain reaction; SD: Standard deviation; Asp: Aspirin; ELISA: Enzyme-linked immunosorbent assay