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  Indian J Med Microbiol
 

Figure 3: Effect of chlorogenic acid on C6 glioma cell death. C6 glioma cells were seeded in 8-well chambers and treated with CA (100 and 300 μM) for 24 h. (a) The cells were stained with 2',7'-dichlorodihydrofluorescein diacetate, Hoechst, and ethidium homodimer. The images were obtained using a fluorescence microscope. (b-d) Bar graphs obtained from the images in panel A, based on the intensity of 2',7'-dichlorodihydrofluorescein diacetate, Hoechst, and ethidium homodimer staining, respectively. The intensity obtained in control cells was considered to be 100%. The data are presented as mean ± standard deviation (n = 3). *P < 0.05 compared with untreated cells. CA: Chlorogenic acid; FBS: Fetal bovine serum; H2DCF-DA: 2',7'-dichlorodihydrofluorescein diacetate; EthD-1: Ethidium homodimer

Figure 3: Effect of chlorogenic acid on C6 glioma cell death. C6 glioma cells were seeded in 8-well chambers and treated with CA (100 and 300 μM) for 24 h. (a) The cells were stained with 2',7'-dichlorodihydrofluorescein diacetate, Hoechst, and ethidium homodimer. The images were obtained using a fluorescence microscope. (b-d) Bar graphs obtained from the images in panel A, based on the intensity of 2',7'-dichlorodihydrofluorescein diacetate, Hoechst, and ethidium homodimer staining, respectively. The intensity obtained in control cells was considered to be 100%. The data are presented as mean ± standard deviation (<i>n</i> = 3). *<i>P</i> < 0.05 compared with untreated cells. CA: Chlorogenic acid; FBS: Fetal bovine serum; H<sub>2</sub>DCF-DA: 2',7'-dichlorodihydrofluorescein diacetate; EthD-1: Ethidium homodimer