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  Indian J Med Microbiol
 

Figure 2: Effect of chlorogenic acid on the migration of fetal bovine serum-stimulated C6 glioma cells. (a) The photographs indicate the migrated cells in the presence or absence of chlorogenic acid (100 and 300 μM) and 10% fetal bovine serum for 90 min. (b) The bar graphs were obtained from panel A. The migration of untreated cells was considered to be 100%. (c) Confluent C6 glioma cell cultures were scratched using a sterilized 200-μL pipette tip and then the cells were cultured in the presence or absence of CA (100 and 300 μM) and 10% fetal bovine serum for 24 h. The photographs indicate the migrated C6 glioma cells in the wound area. The black bold lines indicate distance at time 0. (d) The bar graphs were obtained from panel C. The migration area in untreated cells was considered to be 100%. The data are presented as mean ± standard deviation (n = 3). *P < 0.05 compared with untreated cells. CA: Chlorogenic acid; FBS: Fetal bovine serum

Figure 2: Effect of chlorogenic acid on the migration of fetal bovine serum-stimulated C6 glioma cells. (a) The photographs indicate the migrated cells in the presence or absence of chlorogenic acid (100 and 300 μM) and 10% fetal bovine serum for 90 min. (b) The bar graphs were obtained from panel A. The migration of untreated cells was considered to be 100%. (c) Confluent C6 glioma cell cultures were scratched using a sterilized 200-μL pipette tip and then the cells were cultured in the presence or absence of CA (100 and 300 μM) and 10% fetal bovine serum for 24 h. The photographs indicate the migrated C6 glioma cells in the wound area. The black bold lines indicate distance at time 0. (d) The bar graphs were obtained from panel C. The migration area in untreated cells was considered to be 100%. The data are presented as mean ± standard deviation (<i>n</i> = 3). *<i>P</i> < 0.05 compared with untreated cells. CA: Chlorogenic acid; FBS: Fetal bovine serum