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  Indian J Med Microbiol
 

Figure 1: Effect of chlorogenic acid and Viscum album callus ethanolic extract on C6 glioma cell viability. (a) Callus induction from stem explant of Viscum album. (b) C6 glioma cells were treated with ethanol extract (10, 30, 100, 300, and 1000 μg/mL) for 24 h. Cell viability was tested using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt assay. (c) Liquid chromatography–mass spectrometry spectrum of ethanol extract and the chemical structure of chlorogenic acid. (d) C6 glioma cells were treated with chlorogenic acid (10, 30, 100, and 300 μM) for 24 h. Cell viability was tested using the XTT assay. The cell viability in the fetal bovine serum-only treated group was considered to be 100%. The data are presented as mean ± standard deviation (n = 3). *P < 0.05 compared with untreated cells. CA: Chlorogenic acid; FBS: Fetal bovine serum

Figure 1: Effect of chlorogenic acid and <i>Viscum album</i> callus ethanolic extract on C6 glioma cell viability. (a) Callus induction from stem explant of <i>Viscum album</i>. (b) C6 glioma cells were treated with ethanol extract (10, 30, 100, 300, and 1000 μg/mL) for 24 h. Cell viability was tested using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt assay. (c) Liquid chromatography–mass spectrometry spectrum of ethanol extract and the chemical structure of chlorogenic acid. (d) C6 glioma cells were treated with chlorogenic acid (10, 30, 100, and 300 μM) for 24 h. Cell viability was tested using the XTT assay. The cell viability in the fetal bovine serum-only treated group was considered to be 100%. The data are presented as mean ± standard deviation (<i>n</i> = 3). *<i>P</i> < 0.05 compared with untreated cells. CA: Chlorogenic acid; FBS: Fetal bovine serum