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  Indian J Med Microbiol
 

Figure 1: The inhibitory curve (a) of daidzin prepared in solvents of PBS-T, H-NADES and S-NADES and their calibration curves (b) by icELISA. With fixed concentrations of immobilized cationized ovalbumin-daidzin (2.5 μg/mL) and anti-daidzin mAb (0.2 μg/mL), the absorbance of icELISA reflects the inhibitory effect of free daidzin. A0and A are the absorbance values in the absence and presence of daidzin, respectively. The linearity of determination was 1.95–62.5 ng/mL for H-NADES and 3.90–125 ng/mL for S-NADES, for which standard deviations (n = 3) were indicated as error bars. icELISA: Indirect competitive enzyme-linked immunosorbent assay, S-NADES: Sugar-based natural deep eutectic solvent, H-NADES: Honey natural deep eutectic solvent

Figure 1: The inhibitory curve (a) of daidzin prepared in solvents of PBS-T, H-NADES and S-NADES and their calibration curves (b) by icELISA. With fixed concentrations of immobilized cationized ovalbumin-daidzin (2.5 μg/mL) and anti-daidzin mAb (0.2 μg/mL), the absorbance of icELISA reflects the inhibitory effect of free daidzin. A<sub>0</sub>and A are the absorbance values in the absence and presence of daidzin, respectively. The linearity of determination was 1.95–62.5 ng/mL for H-NADES and 3.90–125 ng/mL for S-NADES, for which standard deviations (<i>n</i> = 3) were indicated as error bars. icELISA: Indirect competitive enzyme-linked immunosorbent assay, S-NADES: Sugar-based natural deep eutectic solvent, H-NADES: Honey natural deep eutectic solvent