Close
  Indian J Med Microbiol
 

Figure 5: Effect of Althea rosea seed extract on apoptotic index by Hoechst 33342 and PI dual staining method. (A) Merged photomicrographs of colonocytes stained with Hoechst 33342 and PI; a: Control, b: 1,2-dimethylhydrazine, c: 1,2-dimethylhydrazine + extract 1, d: 1,2-dimethylhydrazine + Extract 2, e: Extract only. Faint blue color indicated live cells and pink color indicated apoptotic cells. (B) Percentage of both live and apoptotic cells in different groups. Values are mean ± standard deviation of three observations in each group.a P < 0.001 versus control,b P < 0.001 versus control,c P < 0.01 versus control,d P < 0.01 versus 1,2-dimethylhydrazine,e P < 0.05 versus 1,2-dimethylhydrazine. [INSIDE:5] Indicates live cells. [INSIDE:6] Indicates apoptotic cells

Figure 5: Effect of <i>Althea rosea</i> seed extract on apoptotic index by Hoechst 33342 and PI dual staining method. (A) Merged photomicrographs of colonocytes stained with Hoechst 33342 and PI; a: Control, b: 1,2-dimethylhydrazine, c: 1,2-dimethylhydrazine + extract 1, d: 1,2-dimethylhydrazine + Extract 2, e: Extract only. Faint blue color indicated live cells and pink color indicated apoptotic cells. (B) Percentage of both live and apoptotic cells in different groups. Values are mean ± standard deviation of three observations in each group.<sup>a</sup> <i>P</i> < 0.001 versus control,<sup>b</sup> <i>P</i> < 0.001 versus control,<sup>c</sup> <i>P</i> < 0.01 versus control,<sup>d</sup> <i>P</i> < 0.01 versus 1,2-dimethylhydrazine,<sup>e</sup> <i>P</i> < 0.05 versus 1,2-dimethylhydrazine. [INSIDE:5] Indicates live cells. [INSIDE:6] Indicates apoptotic cells