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  Indian J Med Microbiol
 

Figure 3: Water extract of Dendrobium officinale Kimura et Migo increased goblet cell number and Muc5ac expression in conjunctiva of dry eye rats. (a) PAS staining demonstrated significant decrease in conjunctival goblet cells (arrowheads) in model group compared with the normal group. However, the number of goblet cells increased significantly after 8-week treatment of water extract of Dendrobium officinale Kimura et Migo. (b) The number of conjunctival goblet cells was counted under ×200 magnification. (c) Immunohistochemical staining showed that oral administration of water extract of Dendrobium officinale Kimura et Migo increased expression level of Muc5ac in the conjunctival epithelium of dry eye rats significantly. (d) The quantification of Muc5ac expression was expressed as percentage of total area. The representative photos were at ×400 original magnification, scale bar: 50 μm. Data were expressed as mean ± standard error of the mean (n = 5-6), ***P < 0.001 when compared with normal control group,#P < 0.05 when compared with model group

Figure 3: Water extract of <i>Dendrobium officinale</i> Kimura et Migo increased goblet cell number and Muc5ac expression in conjunctiva of dry eye rats. (a) PAS staining demonstrated significant decrease in conjunctival goblet cells (arrowheads) in model group compared with the normal group. However, the number of goblet cells increased significantly after 8-week treatment of water extract of <i>Dendrobium officinale</i> Kimura et Migo. (b) The number of conjunctival goblet cells was counted under ×200 magnification. (c) Immunohistochemical staining showed that oral administration of water extract of <i>Dendrobium officinale</i> Kimura et Migo increased expression level of Muc5ac in the conjunctival epithelium of dry eye rats significantly. (d) The quantification of Muc5ac expression was expressed as percentage of total area. The representative photos were at ×400 original magnification, scale bar: 50 μm. Data were expressed as mean ± standard error of the mean (<i>n</i> = 5-6), ***<i>P</i> < 0.001 when compared with normal control group,<sup>#</sup><i>P</i> < 0.05 when compared with model group