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  Indian J Med Microbiol
 

Figure 1: (a) Amplification of internal transcribed spacer DNA from various Cinnamomum plants by polymerase chain reaction. Polymerase chain reaction was performed using universal primers, TCM-5 and TCM-12, designed based on the sequence of nuclear ribosomal DNA. Polymerase chain reaction conditions were described in the methods and materials. (b) Polymerase chain reaction-restriction fragment length polymorphism analysis. Myl I and Eco RV restriction pattern of a polymerase chain reaction product of internal transcribed spacer DNA from seven Cinnamomum plants were resolved using 3% agarose gel. Lanes 1–5 indicate Cinnamomum osmophloeum; lanes 6–8 indicate C. burmannii; lanes 9–13 indicate Cinnamomum kanehirae, Cinnamomum camphora, Cinnamomum reticulatum, Cinnamomum kotoense, and Cinnamomum cassia, respectively. Lane M: DNA marker

Figure 1: (a) Amplification of internal transcribed spacer DNA from various <i>Cinnamomum</i> plants by polymerase chain reaction. Polymerase chain reaction was performed using universal primers, TCM-5 and TCM-12, designed based on the sequence of nuclear ribosomal DNA. Polymerase chain reaction conditions were described in the methods and materials. (b) Polymerase chain reaction-restriction fragment length polymorphism analysis. <i>Myl</i> I and <i>Eco</i> RV restriction pattern of a polymerase chain reaction product of internal transcribed spacer DNA from seven Cinnamomum plants were resolved using 3% agarose gel. Lanes 1–5 indicate <i>Cinnamomum osmophloeum</i>; lanes 6–8 indicate <i>C. burmannii</i>; lanes 9–13 indicate <i>Cinnamomum kanehirae</i>, <i>Cinnamomum camphora</i>, <i>Cinnamomum reticulatum</i>, <i>Cinnamomum kotoense</i>, and <i>Cinnamomum cassia</i>, respectively. Lane M: DNA marker