Close
  Indian J Med Microbiol
 

Figure 5: Effect of piperine free Piper nigrum extract on apoptosis. KKU-M213 (a and c) and TFK-1 (b and d) cells were treated with Median inhibition concentration concentration of piperine free Piper nigrum extract for 24, 48, 72 and 96 h. Then, the levels of tumor protein p53, B-cell lymphoma 2, Bcl-2-associated X protein and PUMA proteins were investigated using Western blot analysis. Fold change of each protein was measured by densitometry quantitation using ImageJ software and normalized with GAPDH. P < 0.05 of three independent experiments was considered to indicate a statistically significant difference compared to control group (0 h)

Figure 5: Effect of piperine free <i>Piper nigrum</i> extract on apoptosis. KKU-M213 (a and c) and TFK-1 (b and d) cells were treated with Median inhibition concentration concentration of piperine free <i>Piper nigrum</i> extract for 24, 48, 72 and 96 h. Then, the levels of tumor protein p53, B-cell lymphoma 2, Bcl-2-associated X protein and PUMA proteins were investigated using Western blot analysis. Fold change of each protein was measured by densitometry quantitation using ImageJ software and normalized with GAPDH. <i>P</i> < 0.05 of three independent experiments was considered to indicate a statistically significant difference compared to control group (0 h)