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  Indian J Med Microbiol
 

Figure 4: Effect of piperine free Piper nigrum extract on cell growth and cell cycle arrest. KKU-M213 (a and c) and TFK-1 (b and d) cells were treated with Median inhibition concentration concentration of piperine free Piper nigrum extract for 24, 48, 72 and 96 h. Then, the levels of topoisomerase II, AKT8 virus oncogene cellular homolog, avian myelocytomatosis virus oncogene cellular homolog, cyclin D1 and p21 proteins were investigated using Western blot analysis. Fold change of each protein was measured by densitometry quantitation using ImageJ software and normalized with GAPDH. P < 0.05 of three independent experiments was considered to indicate a statistically significant differences compared to control group (0 h)

Figure 4: Effect of piperine free <i>Piper nigrum</i> extract on cell growth and cell cycle arrest. KKU-M213 (a and c) and TFK-1 (b and d) cells were treated with Median inhibition concentration concentration of piperine free <i>Piper nigrum</i> extract for 24, 48, 72 and 96 h. Then, the levels of topoisomerase II, AKT8 virus oncogene cellular homolog, avian myelocytomatosis virus oncogene cellular homolog, cyclin D1 and p21 proteins were investigated using Western blot analysis. Fold change of each protein was measured by densitometry quantitation using ImageJ software and normalized with GAPDH. <i>P</i> < 0.05 of three independent experiments was considered to indicate a statistically significant differences compared to control group (0 h)