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  Indian J Med Microbiol
 

Figure 5: Effects of specific inhibitors on the stimulation of the glucose uptake activity of Astraeus odoratus and Astraeus asiaticus extracts (AO and AA, respectively). L6 myotubes were treated with 100 μg/mL AO and 200 μg/mL AA (a-c, e) or 300 μg/mL AO and 400 ug/mL AA (d) and 50 nM insulin was used as positive control. (a) 3 μM Wortmannin was added after a 1 h treatment; (b and d) 20 μM SB203580 was added after treatment at 4 h; (c) 2 μM cytochalasin B was added after treatment at 4 h; and (e) 3.5 μM cycloheximide was added together with treatment. *P <0.05, **P <0.01 compared the activity without inhibitor versus with inhibitor; n = 3

Figure 5: Effects of specific inhibitors on the stimulation of the glucose uptake activity of <i>Astraeus odoratus </i> and <i>Astraeus asiaticus </i> extracts (AO and AA, respectively). L6 myotubes were treated with 100 μg/mL AO and 200 μg/mL AA (a-c, e) or 300 μg/mL AO and 400 ug/mL AA (d) and 50 nM insulin was used as positive control.  (a) 3 μM Wortmannin was added after a 1 h treatment; (b and d) 20 μM SB203580 was added after treatment at 4 h; (c) 2 μM cytochalasin B was added after treatment at 4 h; and (e) 3.5 μM cycloheximide was added together with treatment. *<i>P</i> <0.05, **<i>P</i> <0.01 compared the activity without inhibitor versus with inhibitor; <i>n</i> = 3