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  Indian J Med Microbiol
 

Figure 5: Effects of SB203580 on Eucheuma cottonii extract-mediated keratinocyte migration. After serum starvation, HaCaT cells were pretreated with SB203580 (5 μM) for 30 min, followed by the Eucheuma cottonii extract (50 μg/mL), and were then incubated for 24 h. Protein samples were analyzed by western blotting with antibodies specific for phospho-p38 mitogen-activated protein kinase (a) and phospho-cAMP response element-binding protein (b). Equal protein loading was confirmed by total p38 and cAMP response element-binding protein expression levels

Figure 5: Effects of SB203580 on <i>Eucheuma cottonii</i> extract-mediated keratinocyte migration. After serum starvation, HaCaT cells were pretreated with SB203580 (5 μM) for 30 min, followed by the <i>Eucheuma cottonii</i> extract (50 μg/mL), and were then incubated for 24 h. Protein samples were analyzed by western blotting with antibodies specific for phospho-p38 mitogen-activated protein kinase (a) and phospho-cAMP response element-binding protein (b). Equal protein loading was confirmed by total p38 and cAMP response element-binding protein expression levels