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Figure 3: Effects of Eucheuma cottonii extract on migration-related signaling pathways. After serum starvation, HaCaT cells were treated with Eucheuma cottonii extract (50 μg/mL) at the indicated time periods and harvested. Protein samples were then examined by western blotting with antibodies specific for phospho-ERK, phospho-Akt (a), phospho-p38 mitogen-activated protein kinase, and phospho-cAMP response element-binding protein (b). Equal protein loading was confirmed by total ERK, Akt, p38 mitogen-activated protein, and cAMP response element-binding protein expression levels |
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