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  Indian J Med Microbiol
 

Figure 4: Effects of LY294002 and SB203580 on Eucheuma cottonii extract-mediated keratinocyte migration. HaCaT cells (5 × 105 cells/well) were seeded into a 6-well plate and serum starved for 24 h. Thereafter, wounds were made in cultures as described in “Materials and Methods.” HaCaT cells were pretreated with LY294002 (20 μM) and SB203580 (5 μM) for 30 min, followed by addition of the Eucheuma cottonii extract (50 μg/mL) and were then incubated for 24 h. (a) Phase contrast images of wound widths were captured using a digital video camera. (b) Quantification of the cell migration rate is shown in the graph. HaCaT cells were maintained for 0 h and 24 h. Data represent the mean ± standard deviation of wound widths in 10 randomly chosen fields expressed as percentages of the control. *P < 0.01 compared to the Eucheuma cottonii extract-treated control

Figure 4: Effects of LY294002 and SB203580 on <i>Eucheuma cottonii</i> extract-mediated keratinocyte migration. HaCaT cells (5 × 10<sup>5</sup> cells/well) were seeded into a 6-well plate and serum starved for 24 h. Thereafter, wounds were made in cultures as described in “Materials and Methods.” HaCaT cells were pretreated with LY294002 (20 μM) and SB203580 (5 μM) for 30 min, followed by addition of the <i>Eucheuma cottonii</i> extract (50 μg/mL) and were then incubated for 24 h. (a) Phase contrast images of wound widths were captured using a digital video camera. (b) Quantification of the cell migration rate is shown in the graph. HaCaT cells were maintained for 0 h and 24 h. Data represent the mean ± standard deviation of wound widths in 10 randomly chosen fields  expressed as percentages of the control. *<i>P</i> < 0.01 compared to the <i>Eucheuma cottonii</i> extract-treated control