Close
  Indian J Med Microbiol
 

Figure 5: Effects of mugunghwa extracts on hyaluronan production and cellular antioxidants/oxidants of keratinocytes. (a) Cell viability was measured by the methylthiazolyldiphenyl-tetrazolium bromide assay. Absorbance was measured at 570 nm using a microplate reader; (b) hyaluronan production (ng/mL) was assessed by measuring the absorbance at 450–540 nm using a microplate reader; HaCaTs were treated with different concentrations of mugunghwa flower, mugunghwa cortex, mugunghwa leaf, mugunghwa root, and mugunghwa seed extracts (10 and 50 μg/mL) for 24 h. Dexamethasone at 10 μg/mL was used as a positive control. (c) The protein levels of antioxidant elements including NAD (p) H:quinone oxidoreductase 1 and heme oxygenase-1 with β-actin as an internal control; (d) analysis of protein expression; (e) intracellular reactive oxygen species production in flow cytometry signals; and (f) analysis of reactive oxygen species production. HaCaTs were irradiated or sham-irradiated with ultraviolet B (100 mJ/cm2), followed by treatment with different concentrations of mugunghwa flower extract (10, 50, and 100 μg/mL) for 24 h. All data are shown as the mean ± standard deviation of at least three independent experiments performed in triplicate

Figure 5: Effects of mugunghwa extracts on hyaluronan production and cellular antioxidants/oxidants of keratinocytes. (a) Cell viability was measured by the methylthiazolyldiphenyl-tetrazolium bromide assay. Absorbance was measured at 570 nm using a microplate reader; (b) hyaluronan production (ng/mL) was assessed by measuring the absorbance at 450–540 nm using a microplate reader; HaCaTs were treated with different concentrations of mugunghwa flower, mugunghwa cortex, mugunghwa leaf, mugunghwa root, and mugunghwa seed extracts (10 and 50 μg/mL) for 24 h. Dexamethasone at 10 μg/mL was used as a positive control. (c) The protein levels of antioxidant elements including NAD (p) H:quinone oxidoreductase 1 and heme oxygenase-1 with β-actin as an internal control; (d) analysis of protein expression; (e) intracellular reactive oxygen species production in flow cytometry signals; and (f) analysis of reactive oxygen species production. HaCaTs were irradiated or sham-irradiated with ultraviolet B (100 mJ/cm<sup>2</sup>), followed by treatment with different concentrations of mugunghwa flower extract (10, 50, and 100 μg/mL) for 24 h. All data are shown as the mean ± standard deviation of at least three independent experiments performed in triplicate