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  Indian J Med Microbiol
 

Figure 2: Expression levels of neuronal nitric oxide synthase mRNA in experimental groups of mice. ΔCt = Ct (nNOS)−Ct (β-actin), where Ct = cycle threshold, β-actin is the endogenous reference gene. Relative neuronal nitric oxide synthase mRNA expression: PΔCt = 2 ΔCt, representing neuronal nitric oxide synthase mRNA levels. Final data in the graph were magnified to 1000 × 2 ΔCt. Data were reported as mean ± standard deviation. A P ≤ 0.05 (two-tailed Student “t” test). Comparisons: *-with Sham; #-with cerebral ischemic reperfusion

Figure 2: Expression levels of neuronal nitric oxide synthase mRNA in experimental groups of mice. ΔCt = Ct (nNOS)−Ct (β-actin), where Ct = cycle threshold, β-actin is the endogenous reference gene. Relative neuronal nitric oxide synthase mRNA expression: PΔCt = 2 ΔCt, representing neuronal nitric oxide synthase mRNA levels. Final data in the graph were magnified to 1000 × 2 ΔCt. Data were reported as mean ± standard deviation. A <i>P ≤</i> 0.05 (two-tailed Student “<i>t</i>” test). Comparisons: *-with Sham; #-with cerebral ischemic reperfusion