Close
  Indian J Med Microbiol
 

Figure 6: Cordyceps militaris fraction inhibits tumor growth in nude mice xenografted with SMMC-7721 cells. (a) Mouse tumor volumes were determined every 4 days during the dosing period. (b) Tumor tissues of nude mice were photographed. (c) Tumor weights of nude mice were measured with an electronic balance. (d) The level of CD31 was measured by IHC analysis (10×). Positive cells were stained brown and negative cells were stained blue.(e) Microvascular IOD was counted by Image-Pro plus Version.(f) Western blots were used to detect protein levels of vascular endothelial growth factor receptor 2, CD31, Akt/p-Akt, and ERK/p-ERK in tumor tissue sections. After 4 × 106 SMMC-7721 cells were inoculated subcutaneously into the flanks of 24 nude mice, the mice were dosed intragastrically with Cordyceps militaris fraction (130 and 260 mg/kg) daily or intraperitoneally with CTX twice a week for 20 days. Data are presented as means ± standard deviation *P < 0.05 and **P < 0.01 versus the model group, n = 6

Figure 6: <i>Cordyceps militaris</i> fraction inhibits tumor growth in nude mice xenografted with SMMC-7721 cells. (a) Mouse tumor volumes were determined every 4 days during the dosing period. (b) Tumor tissues of nude mice were photographed. (c) Tumor weights of nude mice were measured with an electronic balance. (d) The level of CD31 was measured by IHC analysis (10×). Positive cells were stained brown and negative cells were stained blue.(e) Microvascular IOD was counted by Image-Pro plus Version.(f) Western blots were used to detect protein levels of vascular endothelial growth factor receptor 2, CD31, Akt/p-Akt, and ERK/p-ERK in tumor tissue sections. After 4 × 10<sup>6</sup> SMMC-7721 cells were inoculated subcutaneously into the flanks of 24 nude mice, the mice were dosed intragastrically with <i>Cordyceps militaris</i> fraction (130 and 260 mg/kg) daily or intraperitoneally with CTX twice a week for 20 days. Data are presented as means ± standard deviation *<i>P</i> < 0.05 and **<i>P</i> < 0.01 versus the model group, <i>n</i> = 6