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  Indian J Med Microbiol
 

Figure 7: Index of phagocytosis and microbicidal activity (%) of mononuclear phagocytes (RAW 264.7 cells) against Leishmania guyanensis exposed to the standardized geranylgeraniol extract for 1 h. CT, negative control; γ + lipopolysaccharide, positive control containing cells, parasite, 1 ng/mL interferon-γ, and 20 ng/mL lipopolysaccharide; E + G, test 1 (T1) containing cells, parasite, 10 mL standardized extract (6.25 mg/mL); γ + lipopolysaccharide + (E + G), test 2 (T2) containing cells, parasite, 1 ng/mL interferon-γ, 20 ng/mL lipopolysaccharide, and 10 mL standardized extract (6.25 mg/mL). (a) Phagocity activity; (b) % infection macrophages; (c ) Phagocity Index; (d) Number de parasites infective por macrophages. The data are presented as mean standard deviation of three experiments performed in triplicate were compared for significance by Student's test or ANOVA followed by Bonferroni test using the GraphPad Prism software 5.0 (Inc. San Diego, CA, USA). P < 0.05 was considered significant

Figure 7: Index of phagocytosis and microbicidal activity (%) of mononuclear phagocytes (RAW 264.7 cells) against <i>Leishmania guyanensis</i> exposed to the standardized geranylgeraniol extract for 1 h. CT, negative control; γ + lipopolysaccharide, positive control containing cells, parasite, 1 ng/mL interferon-γ, and 20 ng/mL lipopolysaccharide; E + G, test 1 (T1) containing cells, parasite, 10 mL standardized extract (6.25 mg/mL); γ + lipopolysaccharide + (E + G), test 2 (T2) containing cells, parasite, 1 ng/mL interferon-γ, 20 ng/mL lipopolysaccharide, and 10 mL standardized extract (6.25 mg/mL). (a) Phagocity activity; (b) % infection macrophages; (c ) Phagocity Index; (d) Number de parasites infective por macrophages. The data are presented as mean standard deviation of three experiments performed in triplicate were compared for significance by Student's test or ANOVA followed by Bonferroni test using the GraphPad Prism software 5.0 (Inc. San Diego, CA, USA). <i>P</i> < 0.05 was considered significant