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  Indian J Med Microbiol
 

Figure 7: (A) Day 1 – Anti-adipogenesis effect of ethanolic extract of Hydrilla verticillata (test), tributyltin chloride (negative control), orlistat (positive control), and control (untreated) on 3T3 L1 cell lines. (B) Day 4 – Anti-adipogenesis effect of ethanolic extract of Hydrilla verticillata (test), tributyltin chloride (negative control), orlistat (positive control), and control (untreated) on 3T3 L1 cell lines. (a) Normal differentiated 3T3 L1 cell lines, (b) Increase in differentiated cell lines, (c) Total reduction in number of cells, (d) Dead cells. IC50concentrations (concentration of drug causing 50% growth inhibition) of extract was found by plotting log (inhibitor concentration) versus response (% inhibition) from MTT assay using GraphPad Prism software. IC50concentration of extract was found to be 840.91 μg/mL. 1/10th IC50= 84 μg/mL, 1/5th IC50= 160 μg/mL, 1/3rd IC50= 280 μg/mL. IC50: Half-maximal inhibitory concentration; TBT: Tributyltin chloride

Figure 7: (A) Day 1 – Anti-adipogenesis effect of ethanolic extract of <i>Hydrilla verticillata</i> (test), tributyltin chloride (negative control), orlistat (positive control), and control (untreated) on 3T3 L1 cell lines. (B) Day 4 – Anti-adipogenesis effect of ethanolic extract of <i>Hydrilla verticillata</i> (test), tributyltin chloride (negative control), orlistat (positive control), and control (untreated) on 3T3 L1 cell lines. (a) Normal differentiated 3T3 L1 cell lines, (b) Increase in differentiated cell lines, (c) Total reduction in number of cells, (d) Dead cells. IC<sub>50</sub>concentrations (concentration of drug causing 50% growth inhibition) of extract was found by plotting log (inhibitor concentration) versus response (% inhibition) from MTT assay using GraphPad Prism software. IC<sub>50</sub>concentration of extract was found to be 840.91 μg/mL. 1/10<sup>th</sup> IC<sub>50</sub>= 84 μg/mL, 1/5<sup>th</sup> IC<sub>50</sub>= 160 μg/mL, 1/3<sup>rd</sup> IC<sub>50</sub>= 280 μg/mL. IC<sub>50</sub>: Half-maximal inhibitory concentration; TBT: Tributyltin chloride