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  Indian J Med Microbiol
 

Figure 7: Caspase-9 and caspases-3/7 activity in human cervical carcinoma cells after treatment with Kalanchoe blossfeldiana extract. The activity of caspase-9 was measured after 4 h and 24 h of treating the cells with 0.5% dimethyl sulfoxide (control) and Kalanchoe blossfeldiana extract in the concentration range of 2.0–100.0 μg/mL (a). The activity of caspases-3/7 was determined after 24 h of treating the cells with 0.5% dimethyl sulfoxide (b) and Kalanchoe blossfeldiana extract at concentrations of 2.0, 5.0 (c), 10.0, 20.0 (d), 50.0 (e) and 100.0 μg/mL (f). The activities of the executioner caspases in Kalanchoe blossfeldiana-treated cells were compared to the control (g). Each sample was run in triplicate. Error bars represent standard deviations. Significant differences relative to the control are marked with an asterisk “*” (P < 0.05)

Figure 7: Caspase-9 and caspases-3/7 activity in human cervical carcinoma cells after treatment with <i>Kalanchoe blossfeldiana</i> extract. The activity of caspase-9 was measured after 4 h and 24 h of treating the cells with 0.5% dimethyl sulfoxide (control) and <i>Kalanchoe blossfeldiana</i> extract in the concentration range of 2.0–100.0 μg/mL (a). The activity of caspases-3/7 was determined after 24 h of treating the cells with 0.5% dimethyl sulfoxide (b) and <i>Kalanchoe blossfeldiana</i> extract at concentrations of 2.0, 5.0 (c), 10.0, 20.0 (d), 50.0 (e) and 100.0 μg/mL (f). The activities of the executioner caspases in <i>Kalanchoe blossfeldiana</i>-treated cells were compared to the control (g). Each sample was run in triplicate. Error bars represent standard deviations. Significant differences relative to the control are marked with an asterisk “*” (<i>P</i> < 0.05)