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  Indian J Med Microbiol
 

Figure 3: (a) High-performance thin-layer chromatography chromatogram of Costus speciosus for major marker diosgenin after derivatization with detecting reagent in visible range. (b) Overlay spectra of Costus speciosus and diosgenin as reference standard at 440 nm. Tracks from bottom to top follow the order: 2. NBCS-05, 3. NBCS-06, 4. NBCS-07, 5. NBCS-08, 6. NBCS-09, 1,8,14 and 15: Diosgenin. 7. NBCS-10, 9. NBCS-11, 10. NBCS-12, 11. NBCS-13, 12. NBCS-14 and 13. NBCS-15

Figure 3: (a) High-performance thin-layer chromatography chromatogram of <i>Costus speciosus</i> for major marker diosgenin after derivatization with detecting reagent in visible range. (b) Overlay spectra of <i>Costus speciosus</i> and diosgenin as reference standard at 440 nm. Tracks from bottom to top follow the order: 2. NBCS-05, 3. NBCS-06, 4. NBCS-07, 5. NBCS-08, 6.
NBCS-09, 1,8,14 and 15: Diosgenin. 7. NBCS-10, 9. NBCS-11, 10. NBCS-12, 11. NBCS-13, 12. NBCS-14 and 13. NBCS-15