Figure 3: (a) Quantitative analysis of apoptosis-related genes bcl-2 and bax in MCF-7 cells normalized against β-actin. (b) Western blot analysis of bax and bcl-2 expression in MCF-7 cells normalized against β-actin as an internal control. (c and d) Migration analysis using scratch wound healing and transwell assays. (e) Statistical analysis of cell migration by t-tests and one-way analysis of variance. Data are expressed as means ± standard deviation of three independent experiments (n = 3). *P < 0.05 and **P < 0.01 compared with the untreated group