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  Indian J Med Microbiol
 

Figure 5: CAFE did not reduce accumulated lipid in mature 3T3-L1 adipocytes, but reduced lipolysis to a small extent. (a) Analysis of lipid content by oil red O staining in fully differentiated 3T3-L1 adipocytes treated with CAFE. 3T3-L1 cells were differentiated to mature adipocytes followed by treatment with CAFE (50–250 μg/ml) for 4 days. Images represent micrographs of oil red O stained cells. Scale bar: 100 μm. (b) Graph represents absorbance of extracted oil red O from different groups of cells represented in panel A. (c and d). CAFE consistently reduced glycerol release from mature adipocytes to a small extent. Mature adipocytes were treated with CAFE or isoproterenol with (panel D) or without (panel C) adenosine (panel A) and adenosine deaminase treatment. Thereafter, glycerol release in the medium was assayed. Bars represent means ± standard error of the mean n = 3. P values are from t-test. *P < 0.05

Figure 5: CAFE did not reduce accumulated lipid in mature 3T3-L1 adipocytes, but reduced lipolysis to a small extent. (a) Analysis of lipid content by oil red O staining in fully differentiated 3T3-L1 adipocytes treated with CAFE. 3T3-L1 cells were differentiated to mature adipocytes followed by treatment with CAFE (50–250 μg/ml) for 4 days. Images represent micrographs of oil red O stained cells. Scale bar: 100 μm. (b) Graph represents absorbance of extracted oil red O from different groups of cells represented in panel A. (c and d). CAFE consistently reduced glycerol release from mature adipocytes to a small extent. Mature adipocytes were treated with CAFE or isoproterenol with (panel D) or without (panel C) adenosine (panel A) and adenosine deaminase treatment. Thereafter, glycerol release in the medium was assayed. Bars represent means ± standard error of the mean <i>n</i> = 3. <i>P</i> values are from <i>t</i>-test. *<i>P</i> < 0.05