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  Indian J Med Microbiol
 

Figure 4: CAFE reduced expression of key adipogenic effector genes, perilipin 1, and fatty acid synthase. Expression of perilipin 1 and fatty acid synthase transcripts were reduced in CAFE-treated group of induced 3T3-L1 cells. However, CAFE had no significant effect on the mRNA expression of fatty acid-binding protein 4. Cell differentiation and treatments were same as in Figure 3. (a) Representative gel images of amplified adipogenic effector gene transcripts by semi-quantitative reverse transcriptase-polymerase chain reaction. (b) Bars represent normalized expression of fatty acid synthase after 8 days of induction. (c) Normalized expression of perilipin 1 after 8 days of induction. (d) Bars represent normalized expression of fatty acid-binding protein 4 after 8 days of induction. Values in the bar diagrams represent mean ± standard error of the mean n = 2. P values are from t-test. *P < 0.05

Figure 4: CAFE reduced expression of key adipogenic effector genes, perilipin 1, and fatty acid synthase. Expression of perilipin 1 and fatty acid synthase transcripts were reduced in CAFE-treated group of induced 3T3-L1 cells. However, CAFE had no significant effect on the mRNA expression of fatty acid-binding protein 4. Cell differentiation and treatments were same as in Figure 3. (a) Representative gel images of amplified adipogenic effector gene transcripts by semi-quantitative reverse transcriptase-polymerase chain reaction. (b) Bars represent normalized expression of fatty acid synthase after 8 days of induction. (c) Normalized expression of perilipin 1 after 8 days of induction. (d) Bars represent normalized expression of fatty acid-binding protein 4 after 8 days of induction. Values in the bar diagrams represent mean ± standard error of the mean <i>n</i> = 2. <i>P</i> values are from <i>t</i>-test. *<i>P</i> < 0.05