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  Indian J Med Microbiol
 

Figure 3: CAFE-reduced expression of key adipogenesis regulators. (a) Expression of peroxisome proliferator-activated receptor γ 1, peroxisome proliferator-activated receptor γ 2, and C/CAAT enhancer-binding protein alpha were analyzed by semiquantitative reverse transcriptase-polymerase chain reaction. Cells were induced for differentiation ± CAFE (50 and 100 μg/ml) in the same manner as described in Figure 2. (b) Bars represent normalized expression of C/CAAT enhancer binding protein alpha expression after 8 days of adipogenic induction. (c) Bars represent normalized expression of peroxisome proliferator-activated receptor γ 1 after 8 days of induction. (d) Normalized mRNA expression of peroxisome proliferator-activated receptor γ 2 after 8 days of induction. Bars represent mean ± standard error of the mean n = 2. P values are from t-test. *P < 0.05

Figure 3: CAFE-reduced expression of key adipogenesis regulators. (a) Expression of peroxisome proliferator-activated receptor γ 1, peroxisome proliferator-activated receptor γ 2, and C/CAAT enhancer-binding protein alpha were analyzed by semiquantitative reverse transcriptase-polymerase chain reaction. Cells were induced for differentiation ± CAFE (50 and 100 μg/ml) in the same manner as described in Figure 2. (b) Bars represent normalized expression of C/CAAT enhancer binding protein alpha expression after 8 days of adipogenic induction. (c) Bars represent normalized expression of peroxisome proliferator-activated receptor γ 1 after 8 days of induction. (d) Normalized mRNA expression of peroxisome proliferator-activated receptor γ 2 after 8 days of induction. Bars represent mean ± standard error of the mean <i>n</i> = 2. <i>P</i> values are from <i>t</i>-test. *<i>P</i> < 0.05