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  Indian J Med Microbiol
 

Figure 2: CAFE inhibited lipid accumulation in differentiating 3T3-L1 cells. (a) 3T3-L1 cells were differentiated in the presence or absence of CAFE at mentioned doses for 4 days and then maintained in maintenance medium for another 4 days. Thereafter, oil red O staining was performed. Images represent micrographs of oil red O stained cells. Scale bar: 100 μm. (b) Graph represents absorbance of extracted oil red O from different groups of cells represented in panel A. n = 3. Bars represent means ± standard error of the mean. P values are from t-test. *P < 0.05

Figure 2: CAFE inhibited lipid accumulation in differentiating 3T3-L1 cells. (a) 3T3-L1 cells were differentiated in the presence or absence of CAFE at mentioned doses for 4 days and then maintained in maintenance medium for another 4 days. Thereafter, oil red O staining was performed. Images represent micrographs of oil red O stained cells. Scale bar: 100 μm. (b) Graph represents absorbance of extracted oil red O from different groups of cells represented in panel A. <i>n</i> = 3. Bars represent means ± standard error of the mean. <i>P</i> values are from <i>t</i>-test. *<i>P</i> < 0.05