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  Indian J Med Microbiol
 

Figure 1: Spectra of different spectral pre-processing methods for PLS and ANN. (a) Raw specta; (b) constant offset elimination; (c) Min/max normalization; (d) MSC; (e) Straight line subtraction; (f) Standard normal variate transformation; (g) 1st D; (h) 2nd D; (i) 1st D + MSC; (j) 1st D + SLS; (k) 1st D + SNV. 1st D: Fist derivative; 2nd D: Second derivative; MSC: Multiplicative scatter correction; SLS: Straight line subtraction; SNV: Standard normal variate transformation; PLS: Partial least square; ANN: Artificial neural network

Figure 1: Spectra of different spectral pre-processing methods for PLS and ANN. (a) Raw specta; (b) constant offset elimination; (c) Min/max normalization; (d) MSC; (e) Straight line subtraction; (f) Standard normal variate transformation; (g) 1<sup>st</sup> D; (h) 2<sup>nd</sup> D; (i) 1<sup>st</sup> D + MSC; (j) 1<sup>st</sup> D + SLS; (k) 1<sup>st</sup> D + SNV. 1<sup>st</sup> D: Fist derivative; 2<sup>nd</sup> D: Second derivative; MSC: Multiplicative scatter correction; SLS: Straight line subtraction; SNV: Standard normal variate transformation; PLS: Partial least square; ANN: Artificial neural network