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Figure 4: Western blotting analysis was performed to see the effects of different concentrations of ethyl acetate fraction and butanol fraction on PPARγ and C/EBPα proteins expression in 3T3L1 cells (a). 3T3L1 cells were differentiated with or without the presence of Ethyl acetate fraction (5 μg/mL) and butanol fraction (30 μg/mL) of the rhizome of Angiopteris helferiana and mRNA expression of PPARγ and C/EBPα was measured by quantitative realtime polymerase chain reaction EA fraction (b) and Butanol fraction (c) significantly inhibited the expression of mRNA of PPARγ, and C/EBPα. Data shown represent the mean ± standard error of the mean from three independent experiments. Statistical significance was determined relative to a control by the Student's ttest (*P < 0.05; **P < 0.005) 
