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Figure 2: Effect of Osthole on proliferation and differentiation of osteoblast. (ac) Proliferation of osteoblast was inhibited by Osthole at 1d (10^{4} M, 10^{5} M, and 10^{6} M), 2d (10^{4} M, 10^{5} M, and 10^{6} M), and 3d (10^{4} M and 10^{5} M). Data were expressed as mean ± standard deviation (n = 4, oneway ANOVA and SNKq test). (df) Alkaline phosphatase activity of osteoblast was enhanced by Osthole at 1d (10^{4} M), 2d (10^{4} M and 10^{5} M), and 3d (10^{4} M). Data were expressed as mean ± standard deviation (n = 4, oneway ANOVA and SNKq test). (g) The secretion of osteocalcin was promoted by Osthole at 21d (10^{4} M, 10^{5} M, and 10^{6} M). Data were expressed as mean ± standard deviation (n = 8, oneway ANOVA and SNKq test). (h and i) Mineralization of bone matrix was promoted by Osthole at 21d (10^{4} M). Data were expressed as mean ± standard deviation (n = 4, oneway ANOVA and SNKq test). *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001 
