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  Indian J Med Microbiol
 

Figure 2: Effect of Osthole on proliferation and differentiation of osteoblast. (a-c) Proliferation of osteoblast was inhibited by Osthole at 1d (10-4 M, 10-5 M, and 10-6 M), 2d (10-4 M, 10-5 M, and 10-6 M), and 3d (10-4 M and 10-5 M). Data were expressed as mean ± standard deviation (n = 4, one-way ANOVA and SNK-q test). (d-f) Alkaline phosphatase activity of osteoblast was enhanced by Osthole at 1d (10-4 M), 2d (10-4 M and 10-5 M), and 3d (10-4 M). Data were expressed as mean ± standard deviation (n = 4, one-way ANOVA and SNK-q test). (g) The secretion of osteocalcin was promoted by Osthole at 21d (10-4 M, 10-5 M, and 10-6 M). Data were expressed as mean ± standard deviation (n = 8, one-way ANOVA and SNK-q test). (h and i) Mineralization of bone matrix was promoted by Osthole at 21d (10-4 M). Data were expressed as mean ± standard deviation (n = 4, one-way ANOVA and SNK-q test). *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001

Figure 2: Effect of Osthole on proliferation and differentiation of osteoblast. (a-c) Proliferation of osteoblast was inhibited by Osthole at 1d (10<sup>-4</sup> M, 10<sup>-5</sup> M, and 10<sup>-6</sup> M), 2d (10<sup>-4</sup> M, 10<sup>-5</sup> M, and 10<sup>-6</sup> M), and 3d (10<sup>-4</sup> M and 10<sup>-5</sup> M). Data were expressed as mean ± standard deviation (<i>n</i> = 4, one-way ANOVA and SNK-q test). (d-f) Alkaline phosphatase activity of osteoblast was enhanced by Osthole at 1d (10<sup>-4</sup> M), 2d (10<sup>-4</sup> M and 10<sup>-5</sup> M), and 3d (10<sup>-4</sup> M). Data were expressed as mean ± standard deviation (<i>n</i> = 4, one-way ANOVA and SNK-q test). (g) The secretion of osteocalcin was promoted by Osthole at 21d (10<sup>-4</sup> M, 10<sup>-5</sup> M, and 10<sup>-6</sup> M). Data were expressed as mean ± standard deviation (<i>n</i> = 8, one-way ANOVA and SNK-q test). (h and i) Mineralization of bone matrix was promoted by Osthole at 21d (10<sup>-4</sup> M). Data were expressed as mean ± standard deviation (<i>n</i> = 4, one-way ANOVA and SNK-q test). *<i>P</i> < 0.05. **<i>P</i> < 0.01. ***<i>P</i> < 0.001. ****<i>P</i> < 0.0001