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  Indian J Med Microbiol
 

Figure 1: Morphological observation on osteoblast. (a) The second passage of cells isolated from calvarias of neonatal (1 day old) Sprague-Dawley rats. Scale bar: 200 μm. (b) Alizarin-red staining of mineralized nodules produced by mature osteoblasts, which were induced with conditioned medium (α-minimum essential medium + 10% fetal bovine serum + 50 μM ascorbic acid + 10 mM β-glycerophosphate) for 7d. Scale bar: 200 μm. (c) Alkaline phosphatase staining of osteoblasts by diazotization-coupling method. Alkaline phosphatase-positive cells were dyed purple. Scale bar: 80 μm. (d) Alkaline phosphatase staining by diazotization-coupling method and nuclear counterstaining with methyl green. Alkaline phosphatase-positive cytoplasm was light purple while nuclears were green. Scale bar: 40 μm

Figure 1: Morphological observation on osteoblast. (a) The second passage of cells isolated from calvarias of neonatal (1 day old) Sprague-Dawley rats. Scale bar: 200 μm. (b) Alizarin-red staining of mineralized nodules produced by mature osteoblasts, which were induced with conditioned medium (α-minimum essential medium + 10% fetal bovine serum + 50 μM ascorbic acid + 10 mM β-glycerophosphate) for 7d. Scale bar: 200 μm. (c) Alkaline phosphatase staining of osteoblasts by diazotization-coupling method. Alkaline phosphatase-positive cells were dyed purple. Scale bar: 80 μm. (d) Alkaline phosphatase staining by diazotization-coupling method and nuclear counterstaining with methyl green. Alkaline phosphatase-positive cytoplasm was light purple while nuclears were green. Scale bar: 40 μm