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  Indian J Med Microbiol
 

Figure 2: Hepatic immune response to 2-AAF, AFB1, IQ, TRP-P-2, and Lactarius necator protein extract. Rat hepatocyte/immune cell preparations were isolated as described in the methods section. The cell preparations were stimulated with 2-AAF, AFB1, IQ, TRP-P-2, or Lactarius necator at a concentration of 1.5 × 10−3 M of each substance or left untreated for 12, 24, and 48 h (n = 20 for each stimulation). Cell supernatants were subsequently removed for measurement of IFNγ as described in the methods section. Results are presented ± standard deviation. Significant differences compared to the control were determined using Mann–Whitney U-test. P < 0.0001 was considered statistically significant different comparing levels of IFNγ release at 12 and 48 h. Reproduction size at column width. 2-AAF: 2-acetylaminofluoene; AFB1: Aflatoxin B1; AFB1: Aflatoxin B1; IQ: Amino-3-methylimidazo[4,5-f] quinoline; TRP-P-2: 3-amino-1-methyl-5H-pyrido-(4,3-b)-indole

Figure 2: Hepatic immune response to 2-AAF, AFB<sub>1</sub>, IQ, TRP-P-2, and <i>Lactarius necator</i> protein extract. Rat hepatocyte/immune cell preparations were isolated as described in the methods section. The cell preparations were stimulated with 2-AAF, AFB<sub>1</sub>, IQ, TRP-P-2, or <i>Lactarius necator</i> at a concentration of 1.5 × 10<sup>−3</sup> M of each substance or left untreated for 12, 24, and 48 h (<i>n</i> = 20 for each stimulation). Cell supernatants were subsequently removed for measurement of IFNγ as described in the methods section. Results are presented ± standard deviation. Significant differences compared to the control were determined using Mann–Whitney U-test. <i>P</i> < 0.0001 was considered statistically significant different comparing levels of IFNγ release at 12 and 48 h. Reproduction size at column width. 2-AAF: 2-acetylaminofluoene; AFB<sub>1</sub>: Aflatoxin B<sub>1</sub>; AFB1: Aflatoxin B1; IQ: Amino-3-methylimidazo[4,5-f] quinoline; TRP-P-2: 3-amino-1-methyl-5H-pyrido-(4,3-b)-indole