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  Indian J Med Microbiol
 

Figure 2: Effect of the sponge extract on the expression of proinflammatory cytokine transcripts in Michigan cancer foundation-7 Cells Real-time reverse transcription polymerase chain reaction analysis was performed to analyze the expression of eight proinflammatory cytokine genes. (a) Tumor Necrosis factor, alpha (TNF), (b) interferon, gamma (IFNG), (c) interleukin-6 (IL-6), (d) interleukin-1, alpha (IL1A), (e) interleukin-1, beta (IL1B), (f) chemokine (C-C Motif) ligand 2 (CCL2), (g) chemokine (C-X-C Motif) ligand 8 (CXCL8), and (h) chemokine (C-X-C Motif) ligand 10 (CXCL10). The values and error bars represent average and standard deviations of three independent sets of experiments. One-way analysis of variance followed by Dunnett posttest was performed to find out significant difference between control and treatments. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001

Figure 2: Effect of the sponge extract on the expression of proinflammatory cytokine transcripts in Michigan cancer foundation-7 Cells Real-time reverse transcription polymerase chain reaction analysis was performed to analyze the expression of eight proinflammatory cytokine genes. (a) Tumor Necrosis factor, alpha (<i>TNF</i>), (b) interferon, gamma (<i>IFNG</i>), (c) interleukin-6 (<i>IL-6</i>), (d) interleukin-1, alpha (<i>IL1A</i>), (e) interleukin-1, beta (<i>IL1B</i>), (f) chemokine (C-C Motif) ligand 2 (<i>CCL2</i>), (g) chemokine (C-X-C Motif) ligand 8 (<i>CXCL8</i>), and (h) chemokine (C-X-C Motif) ligand 10 (<i>CXCL10</i>). The values and error bars represent average and standard deviations of three independent sets of experiments. One-way analysis of variance followed by Dunnett posttest was performed to find out significant difference between control and treatments. *<i>P</i> ≤ 0.05; **<i>P</i> ≤ 0.01; ***<i>P</i> ≤ 0.001