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Figure 2: Effect of the sponge extract on the expression of proinflammatory cytokine transcripts in Michigan cancer foundation-7 Cells Real-time reverse transcription polymerase chain reaction analysis was performed to analyze the expression of eight proinflammatory cytokine genes. (a) Tumor Necrosis factor, alpha (TNF), (b) interferon, gamma (IFNG), (c) interleukin-6 (IL-6), (d) interleukin-1, alpha (IL1A), (e) interleukin-1, beta (IL1B), (f) chemokine (C-C Motif) ligand 2 (CCL2), (g) chemokine (C-X-C Motif) ligand 8 (CXCL8), and (h) chemokine (C-X-C Motif) ligand 10 (CXCL10). The values and error bars represent average and standard deviations of three independent sets of experiments. One-way analysis of variance followed by Dunnett posttest was performed to find out significant difference between control and treatments. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 |
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