Close  
Figure 2: Effect of the sponge extract on the expression of proinflammatory cytokine transcripts in Michigan cancer foundation7 Cells Realtime reverse transcription polymerase chain reaction analysis was performed to analyze the expression of eight proinflammatory cytokine genes. (a) Tumor Necrosis factor, alpha (TNF), (b) interferon, gamma (IFNG), (c) interleukin6 (IL6), (d) interleukin1, alpha (IL1A), (e) interleukin1, beta (IL1B), (f) chemokine (CC Motif) ligand 2 (CCL2), (g) chemokine (CXC Motif) ligand 8 (CXCL8), and (h) chemokine (CXC Motif) ligand 10 (CXCL10). The values and error bars represent average and standard deviations of three independent sets of experiments. Oneway analysis of variance followed by Dunnett posttest was performed to find out significant difference between control and treatments. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 
