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  Indian J Med Microbiol
 

Figure 4: The effects of capsaicin on the protein expression of p65-nuclear factor-κB, p-IκB, inducible nitric oxide synthase, and cyclooxygenase-2 in BV-2 microglial cells stimulated with lipopolysaccharide. (a) Cells were pre-treated for 2 h with the indicated concentrations of capsaicin and then stimulated for 24 h with lipopolysaccharide (1 μg/mL). Western blot analyses were performed, and representative blots of three independent experiments are shown. Band intensity was normalized to β-actin; the values are presented below each band and the quantification of the respective bots by ImageJ software (NIH, USA). Relative data represent the mean values of three experiments ± standard deviation. Comparisons: aControl versus lipopolysaccharide; blipopolysaccharide versus lipopolysaccharide + Capsaicin (50 μg/mL); clipopolysaccharide versus Capsaicin (100 μg/mL), *P < 0.05. (b) Effect of capsaicin on immunofluorescence analysis of p65-nuclear factor-κB in lipopolysaccharide-activated BV-2 cells. The images were taken at × 40 magnification (Scale bar 50 μM). (c) The expressions of p65-nuclear factor-κB and p-p65-nuclear factor-κB were studied in the nuclear fraction of BV-2 cells. Tubulin expression was studied as internal control. Relative data represent the mean values of three experiments ± standard deviation. Comparisons: aControl versus LPS; blipopolysaccharide versus lipopolysaccharide + Capsaicin (50 μg/mL); clipopolysaccharide versus Capsaicin (100 μg/mL), *P < 0.05

Figure 4: The effects of capsaicin on the protein expression of p65-nuclear factor-κB, p-IκB, inducible nitric oxide synthase, and cyclooxygenase-2 in BV-2 microglial cells stimulated with lipopolysaccharide. (a) Cells were pre-treated for 2 h with the indicated concentrations of capsaicin and then stimulated for 24 h with lipopolysaccharide (1 μg/mL). Western blot analyses were performed, and representative blots of three independent experiments are shown. Band intensity was normalized to β-actin; the values are presented below each band and the quantification of the respective bots by ImageJ software (NIH, USA). Relative data represent the mean values of three experiments ± standard deviation. Comparisons: <sup>a</sup>Control versus lipopolysaccharide; <sup>b</sup>lipopolysaccharide versus lipopolysaccharide + Capsaicin (50 μg/mL); <sup>c</sup>lipopolysaccharide versus Capsaicin (100 μg/mL), *<i>P</i> < 0.05. (b) Effect of capsaicin on immunofluorescence analysis of p65-nuclear factor-κB in lipopolysaccharide-activated BV-2 cells. The images were taken at × 40 magnification (Scale bar 50 μM). (c) The expressions of p65-nuclear factor-κB and p-p65-nuclear factor-κB were studied in the nuclear fraction of BV-2 cells. Tubulin expression was studied as internal control. Relative data represent the mean values of three experiments ± standard deviation. Comparisons: <sup>a</sup>Control versus LPS; <sup>b</sup>lipopolysaccharide versus lipopolysaccharide + Capsaicin (50 μg/mL); <sup>c</sup>lipopolysaccharide versus Capsaicin (100 μg/mL), *<i>P</i> < 0.05