Close
  Indian J Med Microbiol
 

Figure 5: The activation of AMP-activated protein kinase by ethanol extract of Citrus unshiu peel in MCF-7 cells. MCF-7 cells were treated with different concentrations of ethanol extract of Citrus unshiu peel for 24 h (a), or they were pretreated with compound C, which is an AMP-activated protein kinase inhibitor, for 1 h and then treated with 300 mg/m ethanol extract of Citrus unshiu peel for 24 h (b). (a) Equal amounts of cell lysate were resolved with sodium-dodecyl sulfate-polyacrylamide gels, transferred to membranes, and probed with the indicated antibodies. The proteins were visualized using an enhanced chemiluminescence detection system. Actin was used as an internal control. Representative images of at least three independent experiments are shown. (b) The viability of cells was measured with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The data are expressed as the mean ± standard deviation of three independent experiments (*P < 0.05 vs. untreated control; #P < 0.05 vs. ethanol extract of Citrus unshiu peel-treated cells)

Figure 5: The activation of AMP-activated protein kinase by ethanol extract of <i>Citrus unshiu</i> peel in MCF-7 cells. MCF-7 cells were treated with different concentrations of ethanol extract of <i>Citrus unshiu</i> peel for 24 h (a), or they were pretreated with compound C, which is an AMP-activated protein kinase inhibitor, for 1 h and then treated with 300 mg/m ethanol extract of <i>Citrus unshiu</i> peel for 24 h (b). (a) Equal amounts of cell lysate were resolved with sodium-dodecyl sulfate-polyacrylamide gels, transferred to membranes, and probed with the indicated antibodies. The proteins were visualized using an enhanced chemiluminescence detection system. Actin was used as an internal control. Representative images of at least three independent experiments are shown. (b) The viability of cells was measured with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The data are expressed as the mean ± standard deviation of three independent experiments (*<i>P</i> < 0.05 vs. untreated control; <sup>#</sup><i>P</i> < 0.05 vs. ethanol extract of <i>Citrus unshiu</i> peel-treated cells)