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  Indian J Med Microbiol
 

Figure 5: Fluorescent micrographic observations of acridine orange and propidium iodide double-stained HT-29 cells. Cells were treated with different concentration of HFMOL at 24 h time point and stained with AO/PI dye (×100; scale bar, 100 mm). (a) Untreated (control) cells showed normal morphology without any noticeable apoptosis and necrosis features. (b) Early apoptosis features were recognized after treatment with 100 mg/mL, intercalated with acridine orange dye (bright green) with fragmented DNA blebbing and nuclear margination. (c) Late apoptosis characteristically occurs after treatment with 200 mg/mL, positively stained with orange color indicating the hallmark of late apoptosis. HFMOL: Hexane fraction of Moringa oleifera leaves; AO/PT: Acridine orange/propidium iodide

Figure 5: Fluorescent micrographic observations of acridine orange and propidium iodide double-stained HT-29 cells. Cells were treated with different concentration of HFMOL at 24 h time point and stained with AO/PI dye (×100; scale bar, 100 mm). (a) Untreated (control) cells showed normal morphology without any noticeable apoptosis and necrosis features. (b) Early apoptosis features were recognized after treatment with 100 mg/mL, intercalated with acridine orange dye (bright green) with fragmented DNA blebbing and nuclear margination. (c) Late apoptosis characteristically occurs after treatment with 200 mg/mL, positively stained with orange color indicating the hallmark of late apoptosis. HFMOL: Hexane fraction of <i>Moringa oleifera</i> leaves; AO/PT: Acridine orange/propidium iodide