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  Indian J Med Microbiol
 

Figure 4: The invasion rate of HT-29 cells after treatment with diverse dose of HFMOL in invasion assay. (a) HT-29 colon cancer cells were pretreated with numerous doses (100, 150, and 200 mg/mL) of HFMOL. Cells were scratched and treated, and migration was observed and photographed under-invested microscope at 0 and 24 h at ×20 and the closure rate was calculated based on standardized method. (b) The percentage of inhibition by invasive cells was calculated, with untreated cells denoting 100% (×100, scale bar: 100 mm). Results are given as mean ± SD of three independent experiments. **P < 0.01 versus nontreated group. SD: Standard deviation; HFMOL: Hexane fraction of Moringa oleifera leaves

Figure 4: The invasion rate of HT-29 cells after treatment with diverse dose of HFMOL in invasion assay. (a) HT-29 colon cancer cells were pretreated with numerous doses (100, 150, and 200 mg/mL) of HFMOL. Cells were scratched and treated, and migration was observed and photographed under-invested microscope at 0 and 24 h at ×20 and the closure rate was calculated based on standardized method. (b) The percentage of inhibition by invasive cells was calculated, with untreated cells denoting 100% (×100, scale bar: 100 mm). Results are given as mean ± SD of three independent experiments. **<i>P</i> < 0.01 versus nontreated group. SD: Standard deviation; HFMOL: Hexane fraction of <i>Moringa oleifera</i> leaves